Skin inflammation and lipid disorder induced by topical application of Mal f 1.

Abstract

OBJECTED: Mal f 1, the first allergen cloned from Malassezia furfur, has been found to have positive IgE reactivity in sera from patients with skin inflammation. In vitro, it has also been shown to induce maturation of dendritic cells and release inflammatory factors. However, its role in skin lipid homeostasis remains largely unexplored. METHODS: A mouse model of skin inflammation was established by intradermal injection of Mal f 1 into the ear. Metabolomic analyses were used to study the potential mechanisms of Mal f 1-induced skin inflammation. RESULTS: Subcutaneous injection of Mal f 1 can successfully induce skin inflammation, including skin thickening, inflammatory cell infiltration and the increase of various inflammatory factors. Mal f 1 caused significant changes in 102 lipid metabolites compared with the control group, which includes metabolites in arachidonic acid metabolism, glycerophospholipid metabolism, linoleic acid metabolism, and cholesterol biosynthesis. CONCLUSIONS: The present study analyze the characteristics of Mal f 1-induced cutaneous inflammation and the associated disorders in skin lipid metabolism, thereby offering valuable insights for future investigations into the pathophysiology, prevention, and treatment strategies for these conditions.