sp. nov., a rapidly growing species isolated from diseased koi carp,.

Abstract

A rapidly growing nontuberculous mycobacterium was isolated from diseased koi carp in Niigata, Japan, which was identified as representing a novelspecies through whole genome sequence analysis. The bacterial isolates (NGTWS0302, NGTWS1803and NGTWSNA01) were found to belong to the genusthrough phylogenetic analysis using whole genome sequences of mycobacteria species. The bacterial colony was smooth, moist and non-chromogenic on 1% Ogawa medium at 30 °C. In biochemical characteristic tests, the bacterial isolates showed positive reactions for catalase activity, Tween 80 hydrolysis and tellurite reduction. The isolates were sensitive to 2-4 µg mlampicillin, kanamycin and rifampicin. Based on these results, we propose a novelspecies,sp. nov. The type strain is NGTWS1803(=JCM 35117=ATCC TSD-289).