sp. nov., isolated from faeces of heart failure mouse model.

Abstract

Strain TC023, a Gram-positive, long, rod-shaped, spore-forming anaerobe, was isolated from the faeces of a heart failure mouse model. The strain formed greyish-white coloured colonies with a convex elevation on brain-heart infusion medium supplemented with 0.1 % sodium taurocholate, incubated at 37 °C for 2 days. Taxonomic analysis based on the 16S rRNA gene sequence showed that TC023belonged to the genus, and was closely related toMMM721(97.6 %) andMOL361(97.4 %). The whole genome of the strain has a G+C content of 37.3 mol%. The average nucleotide identity and genome-to-genome distance between TC023andMMM721were 77.6 % and 24.3 %, respectively, and those withMOL361were 75.4 % and 24.3 %, respectively. These genotypic, phenotypic, and biochemical analyses indicated that the isolate represents a novel species in the genus, and the namesp. nov. is proposed. The type strain is TC023(RIMD 2002001=TSD 372).