sp. nov., isolated from feline oral cavities.

Abstract

Three bacterial strains, KC07075, KC07079 and KC07084, were isolated from the oral cavity of cats in 2007 in Japan. These strains were Gram-negative rods, exhibited gliding motility, grew in air with 5 % CO, and showed oxidase activity, but not catalase activity. The 16S rRNA gene sequences of the three strains were 100 % identical. The 16S rRNA gene sequence of strain KC07084showed 92.1 and 91.9% identity to the type strains ofand, respectively, and showed 89.3-91.6% identity to otherspecies. The major cellular fatty acids of strain KC07084were iso-C(58.4 %) and summed feature 11 (13.1 %). The G+C content of DNA from strain KC07084was 33.7 mol%, and the genome size was 2.92 Mbp. Strains KC07075, KC07079 and KC07084showed digital DNA-DNA hybridization values (dDDH) values of 99.9 % and average nucleotide identity (ANI) values of 99.98 % with each other, strain KC07084had dDDH values of 18.7-28.2 % and ANI values of 67.12-72.30 % to the type strains of otherspecies. All known species of the genusinhabiting the oral cavity of dogs and cats have catalase activity, but the three strains, including type strain KC07084, lacked catalase activity. These results of the phylogenetic analysis of the 16S rRNA gene sequence, biochemical characteristics, and dDDH and ANI values suggest that strain KC07084represents a novel species. We propose the namesp. nov., with KC07084as the type strain (=JCM 32682=DSM 107252).