Peer-reviewed veterinary case report
Testing eye swabs to diagnose canine visceral leishmaniasis with LAMP
By Isabela C. S. Santos et al.·Published in Tropical Medicine and Infectious Disease·2024·Leishmaniasis Clinical Research and Surveillance Laboratory, Evandro Chagas National Institute of Infectious Diseases (INI), Oswaldo Cruz Foundation Fiocruz, Rio de Janeiro 21040-900, Rio de Janeiro, Brazil, CH·View original on DOAJ →
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Original publication title: Standardization and Evaluation of the LAMP Technique for the Diagnosis of Canine Visceral Leishmaniasis in Conjunctival Swab Samples Using DNA Extracted by a Silica Column and Boiling
- Species:
- dog
Plain-English summary
A group of 54 dogs suspected of having canine visceral leishmaniasis (CVL) were tested using a new diagnostic method called K26-LAMP, which analyzes DNA from conjunctival swabs. Many of these dogs showed mild to severe symptoms, but some had no noticeable signs at all. The K26-LAMP test was found to be quite effective, especially in dogs with severe symptoms, showing a sensitivity of 97.2% when using a specific DNA extraction method. This new technique could be a reliable option for diagnosing CVL in dogs, similar to other established tests.
People also search for: dog leishmaniasis symptoms · canine visceral leishmaniasis diagnosis · K26-LAMP test for dogs
Abstract
The diagnosis of canine visceral leishmaniasis (CVL) presents a challenge due to a variety of non-specific clinical signs. The available tests have low sensitivity. This study aimed to standardize and evaluate the loop-mediated isothermal amplification technique with K26 target (K26-LAMP) for diagnosis of CVL in conjunctival swab (CS) DNA samples extracted through a silica column commercial kit (SW-kit) and boiling (SW-DB) and to compare sensitivity with conventional PCR (kDNA-cPCR) and quantitative real-time PCR (18S-qPCR). Clinical samples of CSs were collected from 54 dogs after reactive serology tests. Positive parasitological and/or histological tests were used as inclusion criteria for a sensitivity analysis. A total of 79.2% (43/54) of dogs without clinical signs or with mild, moderate, or severe clinical signs were included in the study. The sensitivity results of K26-LAMP, kDNA-cPCR, and 18S-qPCR were 72.1%, 81.4%, and 80.5% with the SW-kit and 97.2%, 95.2%, and 57.1% with SW-DB, respectively. In all techniques, the proportion of positives was higher in the group with severe clinical disease, with statistically significant differences in the K26-LAMP and 18S-qPCR techniques being seen with the SW-kit. The results obtained with LAMP for CS samples are promising and its performance is similar to other techniques.
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Search related cases →Original publication on DOAJ: https://doi.org/10.3390/tropicalmed9110277