Peer-reviewed veterinary case report
Stimulation of cells using EGF-coated magnetic beads.
- Journal:
- Science's STKE : signal transduction knowledge environment
- Year:
- 2004
- Authors:
- Kempiak, Stephan J & Segall, Jeffrey E
- Affiliation:
- Department of Anatomy and Structural Biology · United States
Abstract
The ability to determine the spatial localization of signals can assist in defining the regulation of specific signaling pathways within the cell. Localized application of a stimulus, followed by imaging of various proteins or their phosphorylated products, allows visualization of the propagation scales of responses to the stimulus. To obtain a localized stimulus of epidermal growth factor (EGF), we bound the ligand to magnetic beads, which could be rapidly pulled onto cell surfaces by a magnet. This method allows the spatial analysis of EGF-induced signaling pathways.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/14762217/