Peer-reviewed veterinary case report
Synthesis of Fibroma Viral Deoxyribonucleic Acid Complexes in Rabbit Kidney Cells
- Journal:
- American Journal of Veterinary Research
- Year:
- 1975
- Authors:
- Minocha, H. C. & Sahu, S. P.
- Affiliation:
- From the Department of Infectious Diseases, College of Veterinary Medicine, Kansas Agricultural Experiment Station, Kansas State University, Manhattan, Ks 66506.
- Species:
- rabbit
Abstract
SUMMARY Cytoplasmic extracts of primary rabbit kidney cells inoculated with fibroma virus revealed 2 peaks of dna complexes (120S and > 410S) in a linear sucrose gradient. Pulse-chase experiments demonstrated a shift in the gradient profile of lighter complexes toward heavier complexes. Synthesis of dna complexes was inhibited by adding puromycin or actinomycin D. The dna from virus-infected cultures hybridized 7 to 9 times greater with fibroma virus dna than did the dna from noninfected cultures. The dna complexes became increasingly resistant to deoxyribonuclease digestion as a function of time during viral growth cycle and produced tumors in rabbits.
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Search related cases →Original publication: https://doi.org/10.2460/ajvr.1975.36.12.1789