Use of colistin with rifampicin, trimethoprim-sulfamethoxazole and teicoplanin inmouse infection model.

Abstract

Infections with multidrug-resistant Gram-negative bacteria such asare major cause of morbidity and mortality. Colistin is used commonly to treat these infections. In this study, we evaluated the efficacy of different colistin combinations in ainfection mouse model.Anmouse infection model was developed in 150 experimental animals. Treatment groups were as follows: colistin, colistin + rifampicin, colistin + trimethoprim/sulfamethoxazole, colistin + teicoplanin and a control group. The outcome was bacterial burden in the lung and liver tissues. The treatment groups were subdivided into 24-, 48- and 72-h groups.Colistin and combinations reduce theburden significantly in lung and liver tissues compared with the control group. Compared with colistin alone colistin + rifampicin and colistin + TMP-SMX provided significantly better reduction in the bacterial burden.These results may suggest that rifampicin and TMP-SMX combination with colistin may have a potential role in the treatment ofinfections.