Peer-reviewed veterinary case report
Using Oil Red O stain to diagnose liposarcoma in dogs
By Masserdotti, Carlo et al.·Published in Veterinary clinical pathology·2006·Laboratorio Biodiversity-Division Veterinaria, Italy·View original on PubMed →
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Original publication title: Use of Oil Red O stain in the cytologic diagnosis of canine liposarcoma.
- Species:
- dog
Plain-English summary
A group of dogs with tumors were tested to see if a special stain called Oil Red O could help identify liposarcoma, a type of cancer that contains fat cells. The study found that the tumors diagnosed as liposarcoma showed strong positive staining for lipids, while other types of sarcomas did not. This means that using Oil Red O could be a helpful and cost-effective way for vets to distinguish liposarcoma from other similar tumors.
People also search for: dog tumor diagnosis · liposarcoma in dogs · Oil Red O stain for dog cancer
Abstract
BACKGROUND: Oil Red O, a stain commonly used to demonstrate lipid in frozen tissue, also may be used to stain air-dried cytologic specimens. OBJECTIVE: The purpose of this study was to prospectively evaluate the value of Oil Red O in identifying lipid to aid in the differentiation of liposarcomas from other types of sarcoma. METHODS: Twelve tumor specimens from dogs were evaluated. The tumors were included in the study if initial cytologic evaluation indicated a sarcoma, and if histologic confirmation was available. Oil Red O was applied to all cytologic specimens. RESULTS: Tumor specimens were diagnosed histologically as liposarcoma (3 well-differentiated, 1 pleomorphic), hemangiopericytoma (n = 3), fibrosarcoma (n = 3), malignant fibrous histiocytoma (n = 1), and undifferentiated sarcoma (n = 1). Cytologic specimens from all liposarcomas showed strong positive staining of cytoplasmic vacuoles for lipid. Specimens from other sarcomas stained negative for Oil Red O, with the exception of weak, irregular positive staining in 1 hemangiopericytoma. CONCLUSION: Our results suggest that Oil Red O staining may be an easy, inexpensive, and useful diagnostic tool for the differentiation of liposarcoma from other mesenchymal neoplasms.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/16511789/