Peer-reviewed veterinary case report
Using immunocytochemistry to diagnose dog tumors in serosal cavities
By Przeździecki, R & Sapierzyński, R·Published in Polish journal of veterinary sciences·2014·View original on PubMed →
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Original publication title: Using of immunocytochemistry in differential diagnosis of neoplasms of serosal cavities in dogs.
- Species:
- dog
Plain-English summary
A dog with a tumor in the abdominal cavity was diagnosed using a combination of cytology (examining cells) and immunocytochemistry (a lab test that uses antibodies to identify specific proteins). This approach helped veterinarians differentiate between types of tumors, such as carcinomas (a type of cancer), sarcomas, and mesotheliomas (tumors of the lining of the abdomen). In total, 32 tumors were identified in the study, with the tests proving to be effective and minimally invasive. This method can help guide treatment decisions for dogs with similar conditions.
People also search for: dog abdominal tumor diagnosis · dog cancer types · mesothelioma in dogs treatment
Abstract
The presence of tumor within the serosal cavities, often connected with accumulation of serosal effusion, is a quite common problem in the small animal veterinary medicine. The first step in diagnosis of such cases is cytopathological examination. The aim of the present study was to evaluate the usefulness of cytology and immunocytochemistry, using commercially available antibodies (anti-cytokeratin, anti-vimentin, and anti-desmin), in differential diagnosis of malignant tumors located within serosal cavities in dogs. The final cytological diagnosis of carcinoma/adenocarcinoma, sarcoma, and mesothelioma was obtained on the basis of routine cytopathology and immunocytochemistry, and then confirmed by histopathology and immunohistochemistry. Cytoplasmic immunoreactivitiy of normal mesothelid cells and cytoplasmic immunoreactivity of hyperplastic mesothelial cells revealed constant and strong expression of all examined intermediate filaments: cytokeratin, vimentin and desmin. Application of routine cytopathology and immunocytochemistry allowed 32 neoplastic tumors to be detected: 19 cases of carcinomas/adenocarcinomas, 6 cases of sarcomas, 7 cases of mesotheliomas. Immunostaining of cytopathological samples with chosen set of antibodies: anti-cytokeratin, anti-vimentin, anti-desmin is a useful, and low invasive test for differentiation between mesotheliomas and carcinomas/adenocarcinomas in dogs.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/24724483/