Peer-reviewed veterinary case report
Rapid test vs PCR for detecting parvovirus in dogs with diarrhea
By Sundaran S. Tinky et al.·Published in Veterinary World·2015·Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy, Thrissur, Kerala, India; ss4tinky@gmail.com, NZ·View original on DOAJ →
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Original publication title: Utility of a rapid immunochromatographic strip test in detecting canine parvovirus infection compared with polymerase chain reaction
- Species:
- dog
Plain-English summary
A group of 50 dogs with diarrhea suspected of having canine parvovirus (CPV) were tested using two methods: a traditional lab test called polymerase chain reaction (PCR) and a quick test called an immunochromatographic (IC) strip test. The PCR test identified 22 dogs as positive for CPV, while the IC strip test found 18 positive cases. Although the IC test was slightly less sensitive than the PCR, it still proved to be a reliable option for quickly diagnosing CPV in dogs. This means that if your dog has diarrhea and is suspected of having parvovirus, the IC strip test could be a useful, fast way for your vet to check for the infection.
People also search for: dog diarrhea parvovirus test · canine parvovirus symptoms · rapid test for dog parvovirus
Abstract
Aim: The present study was undertaken to detect the presence of canine parvovirus (CPV) in fecal samples of diarrheic dogs by conventional polymerase chain reaction (PCR) and immunochromatographic (IC) strip test and to compare the diagnostic potential of these tests. Materials and Methods: A total of 50 fecal samples collected from diarrheic dogs suspected for CPV infection were subjected to PCR using CPV-555 primer amplifying the gene coding for the VP1 protein. These samples were also tested by IC strip test using a commercial rapid Ag test kit. The results were statistically analyzed using McNemar test. Results: A total of 22 samples (44%) were detected as positive by PCR, which yielded a specific amplicon of 583 bp. In IC strip test, 18 (36%) samples were found to be positive. The sensitivity of the test as compared to PCR was found to be 72.22% and specificity was 92.86%. Positive predictive value and negative predictive value of IC strip test was found to be 88.89% and 81.25%, respectively. Statistical analysis of the results of PCR and IC assay using McNemar test revealed no significant difference (p>0.05). Conclusion: The IC strip test could be employed as a rapid field level diagnostic tool for the diagnosis of canine parvoviral diarrhea.
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Search related cases →Original publication on DOAJ: https://doi.org/10.14202/vetworld.2015.523-526