Peer-reviewed veterinary case report
Antitumor Activity of Rivoceranib Against Canine Mammary Gland Tumor Cell Lines.
- Journal:
- Anticancer research
- Year:
- 2019
- Authors:
- Lee, Jeong-Ha et al.
- Affiliation:
- Department of Veterinary Clinical Science · South Korea
- Species:
- dog
Abstract
BACKGROUND/AIM: Canine mammary gland tumors (CMGTs) are the most common tumors in female dogs. Rivoceranib (also known as apatinib) is a novel anti-angiogenic tyrosine kinase inhibitor that selectively binds to vascular endothelial growth factor receptor-2 (VEGFR2). The aim of this study was to disclose the antitumor effects of rivoceranib on CMGT cell lines. MATERIALS AND METHODS: The direct effects of rivoceranib on CMGT cells in vitro were analyzed by cell proliferation and migration assays. Cell-cycle distribution and apoptotic ratio were analyzed by flow cytometry. Expression levels of phosphorylated VEGFR2 were evaluated by western blot analysis. RESULTS: Rivoceranib treatment significantly reduced the proliferation and migration of CMGT cells in a dose-dependent manner. Flow cytometry results revealed significant increases in G/Gphase arrest and apoptosis proportional to the drug concentration used. Rivoceranib reduced the level of phosphorylated VEGFR2. CONCLUSION: We confirm that rivoceranib exerts antitumor effects on CMGT cells by inhibiting biological functions.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/31570442/