Coexistence of Fosfomycin Resistance Determinantand3 inIsolated from Pets with Urinary Tract Infection in Taiwan.

Abstract

To analyze the characteristics ofand3 inisolated from aspirated and catheterized urine culture specimens of companion pets in Taiwan. A total of 19isolates from pets with urinary tract infection were screened for the presence of,3, andand for the genetic context of them by PCR amplification and sequencing. The transferability, resistance phenotypes, plasmid replicon typing properties and genetic environments of- and/or3-positive strains were characterized. Fiveisolates were positive forand three coharboredand3. Nodeterminant was detected. Transconjugants of3 were successfully acquired, while the acquisition oftransconjugants was failed. The minimum inhibitory concentrations (MICs) of the three3-positive isolates and their transconjugants were ≥256 mg/L, whereas the MICs of the five-positive isolates ranged from 64 mg/L to 256 mg/L. Three plasmid replicons (FrepB,L/M, andHI2) were identified in- and-positiveisolates. Different genetic contexts lay in the downstream region ofand3, respectively. Eight distinct patterns based on the similarity value of more than 80% were typed for all the 8-positive isolates. In conclusion, theconcomitant with3 were found inisolates. The3 not only exhibits stronger activity of inactivating fosfomycin thanbut also possesses stronger potential to spread than. Different genetic backgrounds exist in these- and-positive isolates, and different mobile elements may confer the dissemination ofand.