Peer-reviewed veterinary case report
Development of a highly sensitive gold nanoparticle probe-based assay for bluetongue virus detection.
- Journal:
- Journal of virological methods
- Year:
- 2012
- Authors:
- Yin, Hui-qiong et al.
- Affiliation:
- Institute of Transfusion Medicine · China
Plain-English summary
Researchers have developed a new test that uses tiny gold particles to quickly and accurately detect the bluetongue virus (BTV), which can affect livestock. This test works by capturing a specific part of the virus using special antibodies and then confirming its presence with advanced DNA techniques. It is much more sensitive than older tests, meaning it can find very small amounts of the virus in a sample. This new method not only helps in detecting BTV but can also be adjusted to look for other proteins if needed. Overall, the test shows great promise for improving how we identify this virus.
Abstract
A simple gold nanoparticle (GNP) probe based assay (GNPA) that was modified from a bio-barcode assay (BCA) technique, was developed for ultra-sensitive, rapid detection of the bluetongue virus (BTV) VP7 outer-core protein. This assay captures the VP7 target antigen using the GNP probe coated with anti-VP7 polyclonal antibodies and single-stranded signal DNA. Magnetic microparticle (MMP) probes coated with anti-VP7 monoclonal antibodies were then added to form a sandwich immuno-complex. The single-stranded signal DNA coated onto the GNP probe present in the immuno-complex could then be detected by PCR and real-time fluorescence PCR using a TaqMan probe. The assay has a purified VP7 detection limit of 10(-2)fg/ml which is 8 orders of magnitude greater than that of conventional antigen capture ELISAs and 1 order of magnitude more sensitive than that of a conventional BCA. These results indicate that the GNPA is a highly sensitive method for easy detection of BTV proteins and that it can be modified as needed to measure the presence of other proteins.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22483982/