Peer-reviewed veterinary case report
Using agarose cell block to help diagnose dog mammary tumors
By Diogo Sousa Zanoni et al.·Published in Ciência Rural·2013
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Original publication title: Emprego do cell block de agarose como método complementar no diagnóstico citológico de tumores mamários caninos Employment of cell block of agarose as additional method in the cytological diagnosis of canine mammary tumors
- Species:
- dog
Plain-English summary
A group of 30 female dogs with breast tumors underwent surgery to remove the tumors, and researchers tested a new method called agarose cell block to help diagnose these tumors more accurately. They compared traditional smear tests with the agarose cell block method and found that using both together provided the best results, reducing the chances of false negatives and improving the accuracy of the diagnosis. This study highlights the importance of using advanced diagnostic techniques in veterinary oncology to ensure proper treatment for dogs with breast tumors.
People also search for: dog breast tumor diagnosis · canine mammary tumor treatment · agarose cell block method for dogs
Abstract
Os tumores mamários são neoplasias comuns em diversas espécies, sendo os processos oncológicos de maior incidência em cães. A elevada frequência e agressividade desses processos justificam a busca de métodos diagnósticos e prognósticos rápidos, de custo reduzido e menor invasividade, visando a uma abordagem cirúrgica e terapêutica adequada. O presente estudo avaliou a adequação da utilização da técnica de cell block de agarose como método diagnóstico complementar aos esfregaços tradicionais no diagnóstico desses processos. Para tanto, foram obtidas 51 amostras citológicas de tumores mamários de 30 cadelas que passaram por excisão tumoral no HOVET-UMESP, comparando-se os resultados obtidos a partir dos esfregaços, de cell blocks, e de sua associação (esfregaços cell blocks-1) com o diagnóstico histopatológico. Os melhores resultados foram obtidos mediante a associação dos métodos, reduzindo os resultados falso-negativos e elevando a correlação cito-histológica, reforçando a importância da citologia na rotina oncológica veterinária.<br>The breast tumors are common neoplasms in several species, with high incidence in dogs. The high frequency and aggressiveness of these cases justifies the search for rapid, low cost and less invasive diagnostic methods, seeking for surgical approach and appropriate therapy. This study evaluated the appropriateness of the use of the agarose cell block technique as a diagnostic tool to complement traditional smears in the diagnosis of these processes. Therefore, it was obtained 51 samples from 30 dogs with breast tumors that underwent tumoral excision at the HOVET-UMESP, comparing the results obtained from smears, cell blocks, alone and in association (smears cell blocks-1), with the histopathologic diagnosis. The best results were obtained with the association of smears and cell block analysis, reducing the false negative results and increasing the cyto-histological correlation, reinforcing the importance of the use of cytology in routine veterinary oncology.
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