Peer-reviewed veterinary case report
How well 5 quick tests find leishmaniosis antibodies in dogs
By Hailmann, Regina et al.·Published in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·2026·LMU Small Animal Clinic, Germany·View original on PubMed →
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Original publication title: Evaluation of 5 point-of-care tests for the detection ofantibodies in dogs.
- Species:
- dog
Plain-English summary
A group of 160 dogs that had lived in or traveled to areas where leishmaniosis (a serious disease caused by a parasite) is common were tested for antibodies using five different quick tests. The tests showed varying levels of accuracy, with the Ab test and Uranotest2.0 being the most reliable, detecting antibodies in about 89% of the cases. The FASTest Leish test, while very specific, was less effective at detecting the disease. The Ab test is recommended for screening dogs from these high-risk areas and for confirming infections in dogs showing symptoms.
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Abstract
Canine leishmaniosis is a widespread, potentially fatal disease caused by infection with. We evaluated 5 commercial point-of-care tests (POCTs) for the detection ofantibodies in dog sera and compared their performance with the immunofluorescence assay (IFA) as the reference method. We tested serum samples from 160 dogs that either had lived in or traveled to leishmaniosis-endemic areas using the following immunochromatography POCTs: the trüRapid Leish (Biotech), FASTest Leish (Megacor),Ab (Bionote), and Uranotest2.0 and 3.0 (Uranovet). Practicality, sensitivity, specificity, positive predictive value, and negative predictive value were determined. The prevalence ofantibodies in all dogs was 28.1% as determined by IFA. The highest sensitivity among POCTs was achieved by theAb and the Uranotest2.0 (88.9%), followed by the trüRapid Leish (86.4%), the Uranotest3.0 (84.4%), and the FASTest Leish (48.9%). The highest specificity was achieved by the FASTest Leish (100%) followed by theAb (98.3%), the trüRapid Leish (96.5%), the Uranotest3.0 (94.8%), and the Uranotest2.0 (90.4%). Given its comparatively high sensitivity and specificity, theAb may be considered for screening dogs from endemic areas and for confirminginfection in clinical dogs.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/41655194/