Evaluation of Serum 3-Bromotyrosine Concentrations in Dogs with Steroid-Responsive Diarrhea and Food-Responsive Diarrhea.

Abstract

BACKGROUND: The clinical usefulness of serum 3-BrY concentrations for subclassifying dogs with food-responsive diarrhea (FRD) and steroid-responsive diarrhea (SRD) has not been studied. HYPOTHESIS/OBJECTIVES: To compare serum 3-BrY concentrations in dogs with FRD, dogs with SRD, and healthy control dogs. ANIMALS: 38 dogs with FRD, 14 dogs with SRD, and 46 healthy dogs. METHODS: Prospective study. Measurement of 3-BrY concentration in serum samples was performed by gas chromatography/mass spectrometry. RESULTS: There was no association of peripheral eosinophilia in dogs with FRD, SRD, and healthy control dogs (P = 0.069). There was no significant correlation between peripheral eosinophil counts and serum 3-BrY concentrations (&#x3c1; = -0.15, P = 0.13). Serum 3-BrY concentrations in dogs with SRD (median [range] = 3.27, 0.9-26.23 &#x3bc;mol/L) were significantly higher than in dogs with FRD (median [range] = 0.99, 0.62-8.82 &#x3bc;mol/L; P = 0.007) or in healthy dogs (median [range] = 0.62, 0.62-1.79 &#x3bc;mol/L; P < 0.001). Also, serum 3-BrY concentrations in dogs with FRD were significantly higher than in healthy dogs (P = 0.025). There was no significant correlation between the canine chronic enteropathy clinical activity index and serum 3-BrY concentrations (&#x3c1; = 0.17, P = 0.23). CONCLUSIONS AND CLINICAL IMPORTANCE: Measurement of serum 3-BrY concentrations, but not the peripheral eosinophil count, is helpful for detecting dogs with SRD and FRD.