Peer-reviewed veterinary case report
Gene expression changes in canine mammary tumors in Brazil
By Faro, Thamirys Aline Silva et al.·Published in Anticancer research·2018·Biological Science Institute, Brazil·View original on PubMed →
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Original publication title: Expression Pattern ofand Its Regulators in Canine Mammary Tumors.
- Species:
- dog
Plain-English summary
A study looked at mammary tumors in female dogs, which are the most common type of cancer in this species. Researchers found that certain genes were more active in tumor tissues compared to healthy samples, except for one specific gene called Cdkn2b. This suggests that the other genes could help in diagnosing mammary cancer in dogs. While the study didn't find a link between gene activity and methylation (a chemical change that can affect gene expression), the findings may still be useful for veterinarians in identifying and treating this type of cancer in dogs.
People also search for: dog mammary cancer symptoms · female dog tumor diagnosis · canine cancer gene testing
Abstract
BACKGROUND/AIM: In female dogs, mammary cancer is the most frequent cancer type, accounting for 50% of all tumors affecting these animals. Amongst the commonly altered genes in cancer is the cell-cycle regulator cyclin-dependent kinase inhibitor 2B (Cdkn2b), whose expression is negatively regulated by protein products of BMI1 proto-oncogene (Bmi1), MYC proto-oncogene (Myc) and T-box gene transcription factor 2 (Tbx2) genes. Considering this, the aim of this study was to evaluate the expression pattern of the Cdkn2b gene and these regulators in canine mammary tumors of dogs from Northern Brazil (Belém, Pará). MATERIAL AND METHODS: Gene expression in samples from 33 animals was assessed by real-time polymerase chain reaction. To check the influence of methylation on gene expression, bisulfite sequencing polymerase chain reaction was also performed. RESULTS: All studied genes, except Cdkn2b, were found at increased expression levels in tumor tissue when compared with control samples. No correlation between expression and methylation data was observed. CONCLUSION: Our results suggest these markers may have a diagnostic value in the veterinary clinic.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/30396955/