Peer-reviewed veterinary case report
Detecting Ki-67 in Diff-Quik stained dog mammary tumor smears
By Choi, U S & Kim, D Y·Published in Cytopathology : official journal of the British Society for Clinical Cytology·2011·Department of Veterinary Clinical Pathology, South Korea·View original on PubMed →
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Original publication title: Immunocytochemical detection of Ki-67 in Diff-Quik-stained cytological smears of canine mammary gland tumours.
- Species:
- dog
Plain-English summary
A group of 24 dogs with mammary gland tumors had fine needle aspirate samples taken to check for a protein called Ki-67, which helps determine if a tumor is benign or malignant. The results showed that the Ki-67 test was successful in most samples, allowing vets to distinguish between benign and cancerous tumors with high accuracy. This method can be particularly useful when traditional tissue samples are not available. Overall, using Diff-Quik stained cytology smears can help in assessing the nature of mammary tumors in dogs.
People also search for: dog mammary tumor treatment · Ki-67 test for dog tumors · how to tell if a dog tumor is cancerous
Abstract
OBJECTIVE: To investigate whether Diff-Quik stained fine needle aspirate smears can be used to evaluate Ki-67 expression by immunocytochemistry. METHODS: Both cytological and histological samples were obtained from 24 dogs with spontaneously developed mammary gland tumours. The cytological and histological specimens were examined by Diff-Quik and H&E stains, respectively. After examination, both samples were immunostained using the same Ki-67 antibody. The % Ki-67 values were calculated based on the percentage of positively stained tumour cells per 500 and 1000 tumour cells in cytology and histology specimens, respectively. RESULTS: Ki-67 staining was successful in 17/24 smears (71%) and 19/23 sections (83%). The correlation coefficient between the percentage of Ki-67-positive cells in cytological smears and in the histological sections was 0.677 (P < 0.01). These values were significantly different between histologically benign and malignant tumour groups both in cytology and histology samples (P < 0.001). The threshold value of the percentage of Ki-67-positive cells for distinguishing benign from malignant tumours was set at 4.85% with 90.9% sensitivity and 92.3% specificity by Receiver Operating Characteristic (ROC) curve using histopathology as the gold standard. CONCLUSION: Diff-Quik-stained cytology smears can be used to detect the presence of Ki-67 antigen when histology sections are not available.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/20545725/