Peer-reviewed veterinary case report
Detecting ovarian cancer cells in dog fluid samples using cell block
By Kita, Chiaki et al.·Published in The Journal of veterinary medical science·2022·Shikoku Cytopathological Laboratory, Japan·View original on PubMed →
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Original publication title: Immunohistochemical features of canine ovarian papillary adenocarcinoma and utility of cell block technique for detecting neoplastic cells in body cavity effusions.
- Species:
- dog
Plain-English summary
A 7-year-old female dog with ovarian papillary adenocarcinoma presented with fluid buildup in her abdomen and chest, known as ascites and pleural effusion. Tests showed that the fluid contained cancer cells, which were identified using specific markers. The study found that these cancer cells were positive for certain proteins, helping to distinguish them from normal cells in the fluid. This information can assist veterinarians in diagnosing this type of cancer more accurately. Treatment options may include surgery or chemotherapy, depending on the individual case.
People also search for: dog ovarian cancer symptoms · ascites in dogs treatment · pleural effusion in dogs causes
Abstract
Dogs with ovarian papillary adenocarcinoma occasionally present with ascites and/or pleural effusion. These aspirated fluids often contain a large number of cells, and distinction between neoplastic cells and activated mesothelial cells can be difficult. In this study, 7 cases of canine ovarian papillary adenocarcinoma, including 3 with ascites and pleural effusion, were immunohistochemically examined. Ovarian tumor cells were positive for cytokeratin CAM5.2 (CAM5.2), Wilms' tumor 1 (WT-1) and progesterone receptor (PR) in all 7 cases. A metastatic lesion of the mediastinum in one case was also positive for CAM5.2, WT-1 and PR. Immunohistochemistry on cell blocks obtained from ascites and/or pleural effusion of 2 cases revealed the presence of PR-positive epithelial cells. Whereas, activated mesothelial cells in ascites or pleural effusion collected from dogs without neoplastic lesions were negative for PR. In addition, surface epithelium and subsurface epithelial structures (SES) of normal canine ovaries, that are considered to be the cell of origin for ovarian papillary adenocarcinoma, were also positive for CAM5.2, WT-1 and PR. These results indicate that, together with CAM5.2, WT-1 and PR is a useful diagnostic marker for canine ovarian papillary adenocarcinoma. Expression of PR may be associated with progesterone-dependent nature of canine ovarian papillary adenocarcinoma.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/35110458/