Indole Alkaloids and Phenolic Amides from the Rhizomes of <i>Cimicifuga heracleifolia</i> and Their In Vitro Soluble Epoxide Hydrolase (sEH) Inhibitory Activity.

Abstract

<i>Cimicifuga heracleifolia</i> is a perennial herb that belongs to the Ranunculaceae family. Its dried rhizomes are a source of Cimicifugae Rhizoma, a traditional Chinese medicine used for detoxification, the treatment of febrile diseases, and the prevention of pathogenic invasion. In the present study, a phytochemical investigation of the rhizomes of <i>C. heracleifolia</i> resulted in the isolation of three indole alkaloids (<b>1</b>-<b>3</b>) and seven phenolic amides (<b>4</b>-<b>10</b>), including three new compounds, 6-methoxy-3-(3-methyl-1-oxo-2-butenyl) 1<i>H</i> indole (<b>1</b>), (3<i>R</i>)-1-(<i>β</i>-d-glucopyranosyl)-3-hydroxy-3-(3-methylbutyl)-2-oxindole (<b>3</b>), and <i>N</i>-acetyldopamine-3-<i>O</i>-<i>β</i>-d-allopyranoside (<b>4</b>). Their structures were elucidated using extensive physicochemical and spectroscopic analyses. All the isolated compounds were evaluated for their inhibitory activity against soluble epoxide hydrolase (sEH). The results showed that cimicifugamide A (<b>6</b>) exhibited the most potent inhibitory activity, with an IC₅₀ value of 8.74 μM, followed by cimicifugamide (<b>7</b>), demethoxycimicifugamide (<b>8</b>), and <i>N</i>-<i>trans</i>-feruloyl tyramine (<b>10</b>), with IC₅₀ values ranging from 15.63 to 20.58 μM. Kinetic analysis revealed that compound <b>6</b> inhibited sEH through a non-competitive mechanism.