Molecular profile PCR array of regenerative therapy (PRP, PRF& CpG-ODN) in wound healing of diabetic dogs.

Abstract

This study's aim is to validate differential wound healing genes expression in canine with diabetes using regenerative medicine such as PRP, PRF, and CpG oligodeoxynucleotides (ODN), for these purpose thirty six diabetic dogs were used and subdivided into 6 groups (6 dogs each) with full-thickness incisional wounds, wound healing was evaluated with a focus on histological changes in wound tissue and gene expression after 3 weeks. The molecular mechanism of wound healing responding to different treatments in dogs were done using the new molecular technique RT2 Profiler PCR array (Qiagen), which enables us to view a focused panel of genes (84 genes) responsible for wound healing. CpG-ODN contributes to low cellular infiltration; PRP improves diabetic wound closure rapidly. The study illustrated that the healing rate was higher in the PRP & CpG-ODN. The animal in this group showed a clinically higher contraction rate of the wound area, upregulates 37 genes responsible for wound healing and downregulates ten genes for the same purpose, also marked collagen production were observed with complete epithelialization. Our study concluded that PRP & CpG-ODN was superior to PRF & CpG-ODN, PRP, PRF, CpG-ODN, and the control group, respectively, for enhancing wound healing in diabetic dogs.