Peroxisome proliferator-activated receptor expression in the canine endometrium with cystic endometrial hyperplasia-pyometra complex.

Abstract

The most common uterine diseases in bitches occurring during diestrus are cystic endometrial hyperplasia (CEH) and pyometra. These diseases can coexist as CEH-pyometra complex (CEH-P). Their pathogenesis has not been fully explained. Peroxisome proliferator-activated receptors (PPARs) are important factors regulating mammalian reproductive function and inflammatory processes. Although there is a lack of data concerning the expression of PPARs in the canine endometrium during CEH and CEH-P, we hypothesized that they might be involved in the development of pathological disorders of the canine endometrium. Therefore, the current study was conducted to evaluate and compare PPAR&#x3b1;, PPAR&#x3b4; and PPAR&#x3b3; mRNA expression using quantitative real-time PCR (qPCR) and their immunolocalization using immunohistochemistry (IHC) staining in the endometrium of clinically healthy bitches (control group; n&#xa0;=&#xa0;8) and those with CEH (n&#xa0;=&#xa0;8) or CEH-P (n&#xa0;=&#xa0;8). For quantification, the arithmetic means of all intensities of immunostaining from the cells were measured with the optical density. PPAR&#x3b1;, PPAR&#x3b4; and PPAR&#x3b3; were detected in the luminal epithelium, glandular epithelium and stromal cells. The mRNA transcription of PPAR&#x3b1; was higher in the CEH group than in the control group (p&#xa0;<&#xa0;.05). Additionally, the mRNA expression and immunostaining intensities of PPAR&#x3b4; and PPAR&#x3b3; in the endometrium in the CEH-P group were downregulated relative to those in the control group (p&#xa0;<&#xa0;.05). Moreover, the serum progesterone concentration measured by direct radioimmunoassay was decreased in the CEH-P group compared to the control group (p&#xa0;<&#xa0;.001) and CEH group (p&#xa0;<&#xa0;.05). The obtained results indicate that PPARs are present in the canine endometrium and that their mRNA profile and intensity levels change under pathological conditions such as CEH and CEH-P. This finding may suggest a correlation between changes in the PPAR expression profile and hormonal disturbances, as well as the potential involvement of PPARs in signal transduction during inflammatory processes occurring in the endometrium during CEH-P. These results pave the way to further research into the role of PPARs in the pathogenesis of CEH and CEH-P in female dogs.