Peer-reviewed veterinary case report
Rapid detection of canine parvovirus in feces using monoclonal antibodies and enzyme-linked immunosorbent assay
- Journal:
- American Journal of Veterinary Research
- Year:
- 1984
- Authors:
- Mildbrand, M. M. et al.
- Affiliation:
- From Syngene Products and Research, A TechAmerica Group, Inc; the Diagnostic Laboratory Colorado State University, Fort Collins, CO 80524, and the Clinic of Medicine, School Veterinary Medicine, University Zurich, CH-8057, Zurich, Switzerland. · United States
- Species:
- dog
Abstract
SUMMARY Monoclonal antibodies were used to develop a double antibody enzyme-linked immunosorbent assay for the detection of canine parvovirus (cpv) antigen in fecal samples. The assay was specific for the hemagglutinating protein of cpv and detected as little as 1.5 ng of virus within a 15-minute incubation period. The use of monoclonal antibodies against epitopes on the cpv antigen permitted the simultaneous addition test sample and enzyme-conjugated antibody, thus considerably simplifying the manipulations required for the assay. Results were visually determined without special instrumentation Clinical studies revealed > 95% correlation between enzyme-linked immunosorbent assay results and hemagglutination titers.
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Search related cases →Original publication: https://doi.org/10.2460/ajvr.1984.45.11.2281