Peer-reviewed veterinary case report
Comparison of hemagglutination and competitive enzyme-linked immunosorbent assay procedures for detecting canine parvovirus in feces
- Journal:
- American Journal of Veterinary Research
- Year:
- 1983
- Authors:
- Mathys, A. et al.
- Affiliation:
- From the Clinics of Medicine (Mathys) and Gynecology (Mueller), School of Veterinary Medicine, University of Zurich, CH-8057 Zurich, Switzerland; and the Department of Medicine (Pedersen) and Department of Surgery (Theilen), School of Veterinary Medicine, University of California, Davis, CA 95616. · United States
- Species:
- dog
Abstract
SUMMARY A competitive enzyme-linked immunosorbent assay (elisa) for detection of canine parvovirus (cpv) antigen in fecal samples was developed. Fecal samples were tested by elisa and a direct hemagglutination assay, and the results compared. The tests gave the same results in 83% and 88% of the fecal samples, depending on whether the samples were treated with chloroform. The discrepancies were due to the fact that each test detected virus in certain fecal samples that was not detected by the other. The use of a monoclonal anti-cpv conjugate resulted in a highly specific test. The performance of the elisa as a competitive assay also increased the reproducibility and sensitivity over that which could be obtained from a classic sandwich-type procedure.
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Search related cases →Original publication: https://doi.org/10.2460/ajvr.1983.44.01.152