Peer-reviewed veterinary case report
Rapid Detection of Cat Cystatin C (cCys-C) Using Immuno-Pillar Chips.
- Journal:
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
- Year:
- 2016
- Authors:
- Mohammadi, Saeed et al.
- Affiliation:
- Graduate School of Chemical Sciences and Engineering · Japan
- Species:
- cat
Abstract
We demonstrated a rapid immunoassay for detection of cat cystatin C (cCys-C) which is an important marker for chronic kidney disease in cats, using immuno-pillar chips. The required amount of reagent solution is 200 times smaller than that for the conventional ELISA in the 96-well microplate (0.5 μL versus 100 μL). In addition, the total assay time in the proposed method is more than 12 times shorter than in the conventional method (20 min versus 240 min). The limit of detection in the new method of 3 ng mLis comparable to that of the conventional method (1 ng mL) and it is in the clinically relevant range.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/27941268/