Peer-reviewed veterinary case report
Rapid detection of encephalomyocarditis virus by one-step reverse transcription loop-mediated isothermal amplification method.
- Journal:
- Virus research
- Year:
- 2014
- Authors:
- Yuan, Wanzhe et al.
- Affiliation:
- College of Animal Medicine · China
Plain-English summary
The encephalomyocarditis virus (EMCV) can lead to serious heart issues in young pigs and reproductive problems in mother pigs. Researchers created a new test called the RT-LAMP assay that can quickly and accurately find EMCV in samples. This test is very sensitive, meaning it can detect even tiny amounts of the virus, and it works in just 50 minutes at a steady temperature. The results can be easily seen using a special gel technique. This new method is promising for diagnosing EMCV in both clinical settings and on farms.
Abstract
The encephalomyocarditis virus (EMCV) can cause acute myocarditis in young pigs or reproductive failure in sows. In this study, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to detect EMCV RNA. The RT-LAMP assay was highly sensitive and able to detect 2.2 × 10(-5)ng of EMCV RNA, as no cross-reaction was observed with other viruses. The RT-LAMP assay was conducted in isothermal (62 °C) conditions within 50 min. The amplified products of EMCV could be detected as ladder-like bands using agarose gel electrophoresis. This is the first report to demonstrate the application of a one-step RT-LAMP assay for the detection of EMCV. The sensitive, specific and rapid RT-LAMP assay developed in this study can be applied widely in clinical diagnosis and field surveillance of EMCV.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/24815878/