Peer-reviewed veterinary case report
Tracking cell-free DNA and lysyl oxidase in dogs with transmissible
By Mohamadzaheri, Mona et al.·Published in BMC veterinary research·2022·Department of Clinical Sciences·View original on PubMed →
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Original publication title: Relationship between plasma cell-free DNA changes and lysyl oxidase during the treatment and prognosis of canine transmissible venereal tumors.
- Species:
- dog
Plain-English summary
A group of 15 male dogs with transmissible venereal tumors (TVT) were monitored during treatment with vincristine, a chemotherapy drug. Researchers looked at changes in cell-free DNA (cfDNA) and lysyl oxidase levels in the dogs' blood to see how well the treatment was working. They found that the cfDNA levels changed significantly after the first week of treatment, indicating a response to the drug, while lysyl oxidase levels increased by the fourth week. These findings suggest that measuring cfDNA and lysyl oxidase could help veterinarians monitor the effectiveness of TVT treatment more effectively.
People also search for: dog cancer treatment · transmissible venereal tumor prognosis · vincristine for dog tumors · monitoring dog cancer treatment
Abstract
BACKGROUND: Transmissible venereal tumors (TVT) are a wide range of canine tumors for which there are no effective markers to monitor the therapeutic response in real-time. Circulating biomarkers can be valuable in early cancer diagnosis and prognosis. Accordingly, this study aimed to investigate the significance of the cell-free DNA (cfDNA) and cfDNA integrity index to monitor the response of TVTs to vincristine and compare them with lysyl oxidase activity. Plasma and sera were collected from fifteen male dogs within four weeks before drug administration. The analytical method was mainly based on the quantitative polymerase chain reaction (qPCR) technique for short and long cfDNAs and lysyl oxidase activity was measured in serum. RESULTS: The results of the cfDNA integrity index showed a significant (p < 0.05) difference in the baseline concentration compared to the second and third weeks (with cut-off values of 1.118 and 93.33% specificity). The cfDNA integrity index increased over time due to the reduction of short cfDNAs in the first week after treatment. Lysyl oxidase activity increased during the fourth week (p < 0.001), but there were no significant differences in the other weeks compared to the baseline. The ROC analysis of lysyl oxidase revealed high sensitivity (100%) and specificity (90%) on the second and third weeks compared to the baseline. Multivariate analysis between cfDNA integrity index and lysyl oxidase showed significant correlation (p < 0.05) only in baseline results. CONCLUSIONS: Overall, short cfDNA, the cfDNA integrity index, and lysyl oxidase activity can be proposed as diagnostic biomarkers and putative prognostic candidates in TVT patients. These biomarkers can be combined with cytology to quickly diagnose TVT.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/35189882/