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Peer-reviewed veterinary case report

Using real-time PCR to track Mycoplasma haemofelis infection in a cat

By Braddock, J A et al.·Published in Journal of feline medicine and surgery·2004·Faculty of Veterinary Science, United Kingdom·View original on PubMed

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Original publication title: The use of real-time PCR in the diagnosis and monitoring of Mycoplasma haemofelis copy number in a naturally infected cat.

Species:
cat

Plain-English summary

A 5-year-old male cat was brought in with severe anemia and breathing problems due to a Mycoplasma haemofelis infection. The vet treated him with a blood transfusion and a 6-week course of doxycycline, which helped him recover quickly. However, he also had an allergic airway disease that needed additional treatment with inhaled medications. Tests showed that the infection was successfully cleared, with no signs of the bacteria found in follow-up tests done months later.

People also search for: cat anemia treatment · Mycoplasma haemofelis in cats · cat breathing problems medication

Abstract

A 5-year-old male neutered cat was diagnosed with severe anaemia due to acute Mycoplasma haemofelis infection. Inflammatory respiratory disease was present concurrently. The cat was treated successfully using a fresh transfusion of whole blood and a 6 week course of doxycycline. The patient made a rapid recovery although the allergic airway disease subsequently required specific therapy consisting of inhaled fluticasone and salbutamol. Real-time quantitative PCR assays confirmed the presence of M. haemofelis DNA copies in the blood at presentation. Repeat PCR assays showed a reduction in copy number during treatment and negative PCR results were obtained both 91 and 425 days after presentation. The report describes, for the first time, the use of real-time PCR in the diagnosis and monitoring of natural M. haemofelis copy number, as well as the induction of long-term negative PCR status.

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Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/15135353/