Peer-reviewed veterinary case report
New urine test measures kidney injury marker cystatin B in dogs
By Lyons, Helen et al.·Published in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·2026·IDEXX Laboratories, United States·View original on PubMed →
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Original publication title: Analytical validation of a novel agglutination immunoassay for the quantification of cystatin B in canine and feline urine.
Plain-English summary
A new urine test for dogs and cats can help veterinarians check for kidney injury by measuring a substance called cystatin B. This test is accurate and reliable, making it a useful tool in diagnosing kidney problems. It works well even when other common substances, like medications or proteins, are present in the urine. For the best results, urine samples should be kept cool and tested within a week. This advancement could help pet owners get quicker and more accurate diagnoses for their furry friends' kidney health.
People also search for: dog kidney injury test · cat kidney disease symptoms · cystatin B urine test for pets
Abstract
Urinary cystatin B (uCysB) is a biomarker of kidney injury in dogs and cats. A high-throughput agglutination immunoassay (Idexx Laboratories) was developed for widespread commercial availability of uCysB testing in a reference laboratory setting. We evaluated immunoassay performance and included analyses of precision, accuracy, linearity, interference, analytical specificity, lot-to-lot variation, and stability. CVs from precision studies on the range of 50-500 ng/mL were 0.38-2.53% (canine) and 0.44-3.5% (feline) for within-run precision, and 1.49-5.09% (canine) and 0.65-5.05% (feline) for between-run precision. Accuracy was measured by recovery percentage and was 89-101% (canine) and 91-112% (feline). Amoxicillin, ciprofloxacin, low concentrations of doxycycline, bilirubin, glucose, ketones, RBCs, hemoglobin, cloudiness, lipids, protein, and pH did not affect results. Urinary cystatin A did not cross-react with the uCysB immunoassay. Results of lot-to-lot linear regressions were 0.90-1.07 (slopes) and 0.97-1.00 (coefficient of determination). One or more freeze-thaw cycles and storage at 30°C impacted the immunoassay stability of canine samples but not feline samples under the same conditions. Our results validate this novel agglutination immunoassay for accurate and precise measurement of uCysB in canine and feline urine samples. For optimal immunoassay performance, samples should be kept at 4°C for a maximum of 1 wk. Our uCysB immunoassay is a useful and practical tool to be used in assessing kidney injury in canine and feline patients in the clinical setting.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/41178683/