Peer-reviewed veterinary case report
Detecting bacteria in French Bulldogs and Boxers with granulomatous
By Smith, Carmen R & Miller, Andrew D·Published in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·2024·Department of Population Medicine and Diagnostic Sciences, United States·View original on PubMed →
PetCaseFinder translated the abstract of this peer-reviewed paper into plain English so pet owners can read it. We do not publish original research — every detail traces back to the citation above. How we work →
Original publication title: In situ hybridization to detectin canine granulomatous colitis.
- Species:
- dog
Plain-English summary
A young French Bulldog or Boxer may show symptoms of diarrhea, weight loss, and possibly vomiting due to a condition called granulomatous colitis, which is linked to an abnormal immune response. In a study involving 29 dogs suspected of having this disease, researchers used a special test called in situ hybridization (ISH) to identify harmful bacteria in the intestines. The ISH test confirmed the diagnosis in most cases, showing that it is a reliable method for detecting this condition. Treatment typically involves antibiotics and dietary changes, which can help manage the symptoms and improve the dog's health.
People also search for: dog diarrhea treatment · French Bulldog colitis symptoms · Boxer weight loss causes
Abstract
Canine granulomatous colitis (histiocytic ulcerative colitis) is an uncommon disease, predominantly of young French Bulldogs and Boxer dogs, that manifests from a dysregulated immune response, primarily to adherent-invasive(AIEC). In conjunction with histopathology and periodic acid-Schiff staining, the diagnosis of granulomatous colitis currently relies on fluorescence in situ hybridization (ISH) or immunohistochemistry to identify and localize AIEC organisms within macrophages in the mucosa and/or submucosa. We investigated the utility of ISH forusing formalin-fixed, paraffin-embedded specimens collected from 29 cases of suspected granulomatous colitis. Most confirmed cases of granulomatous colitis were in French Bulldogs (12 of 20; 60%) and Boxers (3 of 20; 15%), and the mean age was 25 ± 6 mo with no sex predilection.ISH signal localized bacterial genetic material within the mucosa in 20 of 29 (69%) cases, supporting the diagnosis. ISH signal was limited to the lumen in 8 of 29 (28%) cases, which did not support the identification of these organisms as AIEC. The remaining case had no hybridization signal, and the diagnosis of granulomatous colitis was not supported. Our results revealed that ISH is a quick and specific detection method that can effectively confirm the diagnosis of canine granulomatous colitis.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/37968864/