Peer-reviewed veterinary case report
Testing new blood tests for sudden kidney injury in dogs
By Davis, Jennifer et al.Ā·Published in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, IncĀ·2020Ā·College of Veterinary Medicine, AustraliaĀ·View original on PubMed ā
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Original publication title: Validation of a commercial magnetic bead-based multiplex assay for 5 novel biomarkers of acute kidney injury in canine serum.
- Species:
- dog
Plain-English summary
A study looked at a new test for dogs to help diagnose acute kidney injury, which can be a serious condition. The test measures five different markers in the blood, but it was found that two of the markers, clusterin and MCP-1, couldn't be reliably measured. The test worked well for one marker, NGAL, showing it could help vets assess kidney health in dogs. However, results for another marker, cystatin C, should be interpreted carefully due to some variability in the readings. Overall, while the test shows promise, it may not be fully reliable for all markers.
People also search for: dog kidney injury symptoms Ā· acute kidney injury test for dogs Ā· NGAL test for dogs
Abstract
Interest is growing in measurement of novel biomarkers for the diagnosis of acute kidney injury. Multiplex assays may provide a rapid and cost-effective way of measurement; however, sparse information is published regarding their use in dogs. We aimed to validate a commercial magnetic bead-based assay for 5 biomarkers: clusterin (Clus), cystatin C (CysC), kidney injury molecule 1 (KIM-1), monocyte chemoattractant protein 1 (MCP-1), and neutrophil gelatinase-associated lipocalin (NGAL). Intra- and inter-assay imprecision, linearity under dilution (LUD), spike recovery (S-R), and hemoglobin interference were evaluated using serum from healthy and diseased dogs. Additionally, the effect of sample type (serum vs. plasma) was investigated. All values for Clus and MCP-1 were outside the assay's measurable range. Intra- and inter-assay precision were acceptable for NGAL (CVs 8.8% and 13.2%, respectively). Regression analysis of LUD and S-R indicated good linearity for CysC and NGAL. Hemolysis did not affect measurement of any biomarker. Measured concentrations of CysC (= 0.018) and NGAL (= 0.015) were significantly lower in sodium citrate plasma compared to serum. We conclude that this magnetic bead-based assay is precise and accurate for NGAL measurement in canine serum. Inappropriate standards for MCP-1 and Clus, and poor accuracy for KIM-1 measurement, suggest that this assay cannot reliably quantify those biomarkers in canine blood. Measurements of CysC in canine blood using this assay must be interpreted with caution given inter-assay imprecision.
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Search related cases āOriginal publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/32627718/